Use of an aminooxy linker for the functionalization of oligodeoxyribonucleotides.
Bioorg Med Chem Lett
; 11(7): 931-3, 2001 Apr 09.
Article
in En
| MEDLINE
| ID: mdl-11294394
ABSTRACT
We describe the preparation of oligonucleotides containing a 5'-linker bearing an aminooxy group. Use of the trityl protecting group for the aminooxy moiety allows purification of the modified oligonucleotide by reverse phase HPLC and cleavage in mild acidic conditions. Derivatization with an aldehydic reporter group is efficient and rapid.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligodeoxyribonucleotides
/
Organophosphorus Compounds
/
Aldehydes
Language:
En
Journal:
Bioorg Med Chem Lett
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2001
Document type:
Article
Affiliation country:
France