Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.
FEBS Lett
; 496(2-3): 147-51, 2001 May 11.
Article
in En
| MEDLINE
| ID: mdl-11356200
ABSTRACT
Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX-2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E-box and each element independently induced higher COX-2 transcription levels than the overlapping CRE/E-box. Transcription factors associated with the CRE binding complex included c-Jun and CRE binding protein and with the E-box binding complex USF-1; their overexpression significantly induced COX-2 transcription. Therefore, both CRE and E-box promoter elements regulate COX-2 transcription in macrophages.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription, Genetic
/
Up-Regulation
/
Prostaglandin-Endoperoxide Synthases
/
Isoenzymes
/
Macrophages
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
FEBS Lett
Year:
2001
Document type:
Article