Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune responses.
Blood
; 97(11): 3498-504, 2001 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-11369643
ABSTRACT
CD100/Sema4D is a 150-kd transmembrane protein that belongs to the semaphorin family. The interaction of CD100 with CD72 is critical for the immune system. In CD100-deficient mice, the production of specific antibodies against T-cell-dependent antigens is severely impaired, but not against T-cell-independent antigens. Here, a functional soluble CD100 protein (sCD100) released from activated lymphocytes is reported. sCD100 was detected in culture supernatants of activated lymphocytes. Either affinity-purified from supernatants of activated T-cells, or produced as a recombinant sCD100 protein consisting of the extracellular region of the mouse CD100 fused to the human IgG1 Fc (CD100-Fc), sCD100 significantly enhanced CD40-induced B-cell responses. Furthermore, sCD100 was detected either in sera of mice immunized with T-cell-dependent antigens, or in sera of MRL/lpr mice, but not in sera of mice immunized with T-cell-independent antigens. A significant correlation was observed between the level of sCD100 and the titer of autoantibodies in the serum of MRL/lpr mice. This study's findings suggest a potential role for sCD100 in immune responses, including production of antibody, and autoimmune diseases. (Blood. 2001;973498-3504)
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
B-Lymphocytes
/
T-Lymphocytes
/
Antigens, CD
/
Semaphorins
Limits:
Animals
Language:
En
Journal:
Blood
Year:
2001
Document type:
Article
Affiliation country:
Japan