Exchange of domain I from Bacillus thuringiensis Cry1 Toxins Influences protoxin stability and crystal formation.
Curr Microbiol
; 43(1): 1-6, 2001 Jul.
Article
in En
| MEDLINE
| ID: mdl-11375655
ABSTRACT
Influence of domain I exchange on the stability and production of Bacillus thuringiensis Cry1 protoxins as well as on the shape of inclusion and toxicity to Spodoptera exigua and Plutella xylostella larvae was investigated. Chimeric genes were prepared by exchanging the regions coding for domain I between Cry1Aa, Cry1Ab, Cry1Ac, Cry1C, and Cry1E. The AcCC chimera accumulated into bipyramidal inclusion bodies, whereas CEE produced round-shaped inclusion bodies, and ECC and AaEE protoxins produced small granules. AbEE and EAaAa did not produce any inclusion body and were visualized by immunodetection only. AcCC, CEE, ECC, and AaEE were stable to trypsin, whereas AbEE and EAaAa were not. Bioassays showed that the chimeras were not toxic in vivo. However, S. exigua larvae fed with the activated AcCC toxin displayed a lower growth rate.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacillus thuringiensis
/
Bacterial Proteins
/
Bacterial Toxins
/
Endotoxins
Limits:
Animals
Language:
En
Journal:
Curr Microbiol
Year:
2001
Document type:
Article
Affiliation country:
France