A Pseudomonas pyocin typing method applicable to the clinical laboratory.
Am J Med Technol
; 45(8): 688-91, 1979 Aug.
Article
in En
| MEDLINE
| ID: mdl-114048
ABSTRACT
A new pyocin typing method for the identification of specific strains of Pseudomonas aeruginosa is described. The method involves a bilayer agar technique in which the pyocin produced by the organism diffuses from the top agar layer number 2 through a filter paper into the bottom agar layer number 1. The filter paper permits the removal of agar layer number 2 and exposes layer number 1 which is then streaked with the indicator strains. Pyocin typing of fifty clinical isolates using both the bi-layer method and the scrap-chloroform reference method of Gillies and Govan produced identical typing results. A total of 375 clinical isolates has been typed by this inexpensive and time-saving bi-layer agar method.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas aeruginosa
/
Pyocins
/
Bacteriocins
Language:
En
Journal:
Am J Med Technol
Year:
1979
Document type:
Article