Efficient and heritable functional knock-out of an adult phenotype in Drosophila using a GAL4-driven hairpin RNA incorporating a heterologous spacer.

Piccin, A; Salameh, A; Benna, C; Sandrelli, F; Mazzotta, G; Zordan, M; Rosato, E; Kyriacou, C P; Costa, R

Piccin, A; Salameh, A; Benna, C; Sandrelli, F; Mazzotta, G; Zordan, M; Rosato, E; Kyriacou, C P; Costa, R.

Affiliation

Piccin A; Dipartimento di Biologia, Università di Padova, via Ugo Bassi 58/B, 35131 Padova, Italy.

Nucleic Acids Res ; 29(12): E55-5, 2001 Jun 15.

Article in En | MEDLINE | ID: mdl-11410678

Subject(s)

Drosophila Proteins; Drosophila melanogaster/genetics; Fungal Proteins/metabolism; Gene Silencing; Insect Proteins/genetics; Nucleic Acid Conformation; RNA, Double-Stranded/metabolism; Saccharomyces cerevisiae Proteins; Transcription Factors/metabolism; Transgenes/genetics; Animals; Animals, Genetically Modified; Crosses, Genetic; DNA-Binding Proteins; Drosophila melanogaster/embryology; Female; Fungal Proteins/genetics; Genetic Vectors/genetics; Male; Phenotype; Pigmentation/genetics; Promoter Regions, Genetic/genetics; RNA, Double-Stranded/biosynthesis; RNA, Double-Stranded/chemistry; RNA, Double-Stranded/genetics; Transcription Factors/genetics; Transformation, Genetic

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / RNA, Double-Stranded / Fungal Proteins / Transgenes / Insect Proteins / Gene Silencing / Saccharomyces cerevisiae Proteins / Drosophila Proteins / Drosophila melanogaster / Nucleic Acid Conformation Limits: Animals Language: En Journal: Nucleic Acids Res Year: 2001 Document type: Article Affiliation country: Italy Country of publication: United kingdom

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