Factors involved in the time course of response to acetylcholine in mesenteric arteries from spontaneously hypertensive rats.
Eur J Pharmacol
; 423(1): 47-55, 2001 Jun 29.
Article
in En
| MEDLINE
| ID: mdl-11438306
ABSTRACT
The time course of the response to prolonged application of acetylcholine in mesenteric arteries from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar Kyoto rats (WKY) was compared. Only a relaxing response, which was blocked by N(omega)-nitro-L-arginine (L-NOARG), was observed after the prolonged application of a low concentration of acetylcholine (10(-8) M) in both preparations; the response was impaired in SHRSP preparations. Prolonged application of a high concentration of acetylcholine (10(-5) M) induced a second contractile response after a first relaxing response in SHRSP preparations under basal conditions and in WKY preparations in the presence of L-NOARG. This contractile response was attenuated by indomethacin. In the presence of a combination of apamin and charybdotoxin, the relaxing response to the high concentration of acetylcholine was reduced and a contractile response, which was abolished by indomethacin, appeared. In the presence of all of these blockers, a contractile response, which was blocked by cyclo(D-alpha-aspartyl-L-propyl-D-valyl-L-leucyl-D-tryptophyl) (BQ-123), was observed in preparations from WKY but not in preparations from SHRSP. Results indicate that prolonged application of acetylcholine in rat mesenteric arteries induces the release of endothelium-derived relaxing, contracting, hyperpolarizing factors and endothelin-1, and that the mode of action differs between preparations from WKY and SHRSP.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Vasoconstriction
/
Vasodilator Agents
/
Acetylcholine
/
Hypertension
/
Mesenteric Arteries
Limits:
Animals
Language:
En
Journal:
Eur J Pharmacol
Year:
2001
Document type:
Article
Affiliation country:
Japan