PAF receptor and PAF acetylhydrolase expression in the endosalpinx of the human Fallopian tube: possible role of embryo-derived PAF in the control of embryo transport to the uterus.
Hum Reprod
; 16(8): 1583-7, 2001 Aug.
Article
in En
| MEDLINE
| ID: mdl-11473946
ABSTRACT
BACKGROUND:
Prostaglandin-E(2) and platelet-activating factor (PAF) are embryonic-derived signals that time embryo passage into the uterus in the mare and hamster respectively. PAF-like activity is detectable in the spent media of preimplantation human embryos and it has been suggested that PAF may be the embryonic signal that controls embryo transport to the uterus in our species. The actions of PAF are regulated at the level of its synthesis and degradation as well as the expression of a specific cell surface receptor (PAFr). The enzyme PAF acetylhydrolase (PAF-AH) degrades PAF. This study was undertaken to examine whether or not PAFr and PAF-AH are expressed in the human Fallopian tube and to identify the cell types in which they are expressed.METHODS:
The presence of PAFr mRNA in tissue extracts was investigated using reverse transcription-polymerase chain reaction. We amplified the predicted amplicon for PAFr mRNA from RNA samples extracted from Fallopian tubes. The expression of PAF-AH was detected by Western blot and the localization of PAFr and PAF-AH proteins was detected by immunohistochemistry.RESULTS:
Utilizing antibodies against PAFr and PAF-AH, co-localization of the two proteins in the epithelium and stromal cells were demonstrated.CONCLUSIONS:
These observations show that the human Fallopian tube expresses PAFr and PAF-AH at a location compatible with the proposed paracrine role of early embryo-derived PAF.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipases A
/
Uterus
/
Platelet Activating Factor
/
Platelet Membrane Glycoproteins
/
Receptors, Cell Surface
/
Receptors, G-Protein-Coupled
/
Embryo, Mammalian
/
Fallopian Tubes
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Hum Reprod
Journal subject:
MEDICINA REPRODUTIVA
Year:
2001
Document type:
Article
Affiliation country:
Chile