Development and mapping of microsatellites from a microdissected BTA 11-specific DNA library.
Anim Genet
; 32(3): 152-5, 2001 Jun.
Article
in En
| MEDLINE
| ID: mdl-11493264
ABSTRACT
A chromosome-specific library was developed for Bos taurus autosome 11 by chromosome microdissection and microcloning using a bovine primary fibroblast culture, obtained from a t(X;23) heifer, that spontaneously developed a translocation chromosome involving bovine chromosome 11. The library was screened using (AC)12 oligos, positive clones selected, sequenced and primers developed to generate bovine chromosome 11-specific microsatellite markers. This study suggests that chromosome-specific libraries have great potential for development of microsatellite markers for the construction of marker-saturated linkage maps for each chromosome.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Translocation, Genetic
/
X Chromosome
/
Cattle
/
Chromosome Mapping
/
Microsatellite Repeats
Limits:
Animals
Language:
En
Journal:
Anim Genet
Journal subject:
GENETICA
/
MEDICINA VETERINARIA
Year:
2001
Document type:
Article
Affiliation country:
United States