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Effects of hypertonic saline on expression of human polymorphonuclear leukocyte adhesion molecules.
Thiel, M; Buessecker, F; Eberhardt, K; Chouker, A; Setzer, F; Kreimeier, U; Arfors, K E; Peter, K; Messmer, K.
Affiliation
  • Thiel M; Department of Anesthesiology and the. Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-Universität, Munich, Germany. manfried.thiel@ana.med.uni-muenchen.de
J Leukoc Biol ; 70(2): 261-73, 2001 Aug.
Article in En | MEDLINE | ID: mdl-11493618
Hypertonic saline prevents vascular adherence of neutrophils and ameliorates ischemic tissue injury. We hypothesized that hypertonic saline attenuates N-formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated expression of adhesion molecules on human polymorphonuclear leukocytes (PMNLs). fMLP-stimulated up-regulation of beta2-integrins was diminished by hypertonic saline but not by hypertonic choline chloride-, mannitol-, or sucrose-modified Hanks' buffered salt solution. Shedding of L-selectin was decreased by hypertonic saline and choline chloride but not by hypertonic mannitol or sucrose. When the effects of hypertonic sodium chloride- and choline chloride-modified media were compared, neither solution affected fMLP-receptor binding but both equally inhibited fMLP-stimulated increase in intracellular calcium, ionophore A23187, and phorbol myristate acetate (PMA)-stimulated numerical up-regulation of beta2-integrins. Analysis of mitogen-activated protein (MAP) kinases p38 and p44/42 for phosphorylation revealed that hypertonic solutions did not differ in preventing fMLP-stimulated increases in phospho-p38 and phospho-p44/42. Resting PMNLs shrunk by hypertonic saline increased their volume during incubation and further during chemotactic stimulation. Addition of amiloride further enhanced inhibition of up-regulation of beta2-integrins. No fMLP-stimulated volume changes occurred in PMNLs exposed to hypertonic choline chloride, resulting in significant cell shrinkage. Results suggest a sodium-specific inhibitory effect on up-regulation of beta2-integrins of fMLP-stimulated PMNLs, which is unlikely to be caused by alterations of fMLP receptor binding, decrease in cytosolic calcium, attenuation of calcium or protein kinase C-dependent pathways, suppression of p38- or p44/42 MAP kinase-dependent pathways, or cellular ability to increase or decrease volumes.
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Collection: 01-internacional Database: MEDLINE Main subject: Saline Solution, Hypertonic / Cell Adhesion Molecules / Neutrophils Limits: Humans Language: En Journal: J Leukoc Biol Year: 2001 Document type: Article Affiliation country: Germany Country of publication: United kingdom
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Collection: 01-internacional Database: MEDLINE Main subject: Saline Solution, Hypertonic / Cell Adhesion Molecules / Neutrophils Limits: Humans Language: En Journal: J Leukoc Biol Year: 2001 Document type: Article Affiliation country: Germany Country of publication: United kingdom