Regulation of CSF1 promoter by the SWI/SNF-like BAF complex.
Cell
; 106(3): 309-18, 2001 Aug 10.
Article
in En
| MEDLINE
| ID: mdl-11509180
ABSTRACT
The mammalian BAF complex regulates gene expression by modifying chromatin structure. In this report, we identify 80 genes activated and 2 genes repressed by the BAF complex in SW-13 cells. We find that prior binding of NFI/CTF to the NFI/CTF binding site in CSF1 promoter is required for the recruitment of the BAF complex and the BAF-dependent activation of the promoter. Furthermore, the activation of the CSF1 promoter requires Z-DNA-forming sequences that are converted to Z-DNA structure upon activation by the BAF complex. The BAF complex facilitates Z-DNA formation in a nucleosomal template in vitro. We propose a model in which the BAF complex promotes Z-DNA formation which, in turn, stabilizes the open chromatin structure at the CSF1 promoter.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Nuclear Proteins
/
Gene Expression Regulation
/
Macrophage Colony-Stimulating Factor
/
Promoter Regions, Genetic
/
DNA-Binding Proteins
Type of study:
Prognostic_studies
Language:
En
Journal:
Cell
Year:
2001
Document type:
Article
Affiliation country:
United States