Efficient 13C/15N double labeling of the avirulence protein AVR4 in a methanol-utilizing strain (Mut+) of Pichia pastoris.

van den Burg, H A; de Wit, P J; Vervoort, J

van den Burg, H A; de Wit, P J; Vervoort, J.

Affiliation

van den Burg HA; Laboratory of Biochemistry, Wageningen University, The Netherlands.

J Biomol NMR ; 20(3): 251-61, 2001 Jul.

Article in En | MEDLINE | ID: mdl-11519748

Subject(s)

Carbon Isotopes/metabolism; Cladosporium/metabolism; Fungal Proteins/metabolism; Isotope Labeling/methods; Methanol/metabolism; Nitrogen Isotopes/metabolism; Pichia/metabolism; Ammonium Sulfate/metabolism; Cost-Benefit Analysis; Culture Media; Fermentation; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/isolation & purification; Glycerol/metabolism; Hydrogen-Ion Concentration; Hydroxides/pharmacology; Isotope Labeling/economics; Mitochondrial Proteins; Oxidoreductases/genetics; Oxidoreductases/metabolism; Pichia/genetics; Pichia/growth & development; Plant Proteins; Potassium Compounds/pharmacology; Transformation, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Fungal Proteins / Carbon Isotopes / Cladosporium / Methanol / Isotope Labeling / Nitrogen Isotopes Type of study: Evaluation_studies Language: En Journal: J Biomol NMR Journal subject: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM / MEDICINA NUCLEAR Year: 2001 Document type: Article Affiliation country: Netherlands

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