Sequence analysis of the nucleocapsid and phosphoprotein genes of avian pneumoviruses circulating in the US.
Virus Res
; 79(1-2): 15-25, 2001 Nov 05.
Article
in En
| MEDLINE
| ID: mdl-11551642
ABSTRACT
Avian pneumovirus (APV) has recently been described as the cause of a new respiratory syndrome in turkey flocks in the United States. We here describe the complete sequence of the nucleocapsid (N) and phosphoprotein (P) genes of this emerging APV (APV/US). Our results show 59 and 61% nucleotide sequence identity of the APV/US N gene with N genes of previously described European APV subgroups A and B, respectively. The P gene of APV/US showed only 53% nucleotide sequence identity with the ortholog from APV subgroup A. Phylogenetic analyses of both N and P genes clearly demonstrate that the APV/US lineage is evolutionarily related but distinct from European APVs. Moreover, sequence analysis of the N and P genes from two laboratory adapted isolates of APV/US (APV/MN-1a and APV/MN-1b) and from ten clinical samples from APV-infected turkeys suggests only modest level of amino acid divergence in the N (0-0.3%) and P (0-1.4%) proteins. Taken together, the results of this study indicate support that APV/US represents a new subgroup (subgroup C) of APV and show that there is limited heterogeneity in the N and P genes of APV/US isolates.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Viral Proteins
/
Pneumovirus
/
Nucleocapsid
Limits:
Animals
/
Humans
Country/Region as subject:
America do norte
Language:
En
Journal:
Virus Res
Journal subject:
VIROLOGIA
Year:
2001
Document type:
Article
Affiliation country:
United States