RecA protein filaments disassemble in the 5' to 3' direction on single-stranded DNA.
J Biol Chem
; 276(49): 45740-3, 2001 Dec 07.
Article
in En
| MEDLINE
| ID: mdl-11574550
ABSTRACT
RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5' to 3' direction.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rec A Recombinases
/
DNA, Single-Stranded
/
DNA-Binding Proteins
Language:
En
Journal:
J Biol Chem
Year:
2001
Document type:
Article
Affiliation country:
United States