Molecular distribution sensing in a fluorescence resonance energy transfer based affinity assay for glucose.
Spectrochim Acta A Mol Biomol Spectrosc
; 57(11): 2245-54, 2001 Sep 14.
Article
in En
| MEDLINE
| ID: mdl-11603841
ABSTRACT
A newly developed method for determining molecular distribution functions is applied to a widely researched glucose affinity sensor. The reduction in fluorescence resonance energy transfer (FRET) to a malachite green (MG)-dextran complex from allophycocyanin (APC) bound to concanavalin A (ConA) due to displacement of the complex by glucose from ConA provides the basis of the assay. The higher sensitivity and specificity of a new approach to fluorescence decay analysis, over the methods based on conventional Forster-type models, is demonstrated and critical parameters in competitive binding FRET sensing derived.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spectrometry, Fluorescence
/
Glucose
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2001
Document type:
Article
Affiliation country:
United kingdom