[The myc oncogene and transdifferentiation of the retinal pigment epithelium]. / Oncogène myc et transdifferenciation de l'épithélium pigmentaire de la rétine.
J Soc Biol
; 195(2): 107-13, 2001.
Article
in Fr
| MEDLINE
| ID: mdl-11723821
ABSTRACT
The retinal pigment epithelium (RPE) develops from the same sheet of neuroepithelium as the neuroretina. When infected with MC29, a v-myc expressing virus, the RPE cells can be induced to transdifferentiate and to take a neuroretinal epithelium fate. After a PCR-based differential screening from these cells we have identified three genes of interest. Qath5, a quail basic helix-loop-helix (bHLH) gene that is closely related to the Drosophila atonal, and whose expression is found in the developing neuroretina. A Chx10-related homeobox gene also expressed in the developing neuroretina and HuD, a RNA-binding protein not expressed in the RPE but expressed during neurogenesis. Beside these genes whose function is involved in regulating neuronal differentiation myc also induced a transient Mitf expression. Mitf is expressed in the entire optic cup, later restricted to the pigmented retina. Mitf is involved in the regulation of the pigmented differentiation. We conclude that v-myc can reverse the RPE to the bipotential retinal primordia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pigment Epithelium of Eye
/
Genes, myc
/
Oncogene Protein p55(v-myc)
/
Growth Substances
/
Alpharetrovirus
/
Zebrafish Proteins
/
Eye Proteins
Type of study:
Prognostic_studies
Language:
Fr
Journal:
J Soc Biol
Journal subject:
BIOLOGIA
Year:
2001
Document type:
Article