Antigenic determinants of bovine serum albumin.
Int Arch Allergy Immunol
; 126(3): 188-95, 2001 Nov.
Article
in En
| MEDLINE
| ID: mdl-11752875
ABSTRACT
BACKGROUND:
Bovine serum albumin (BSA) is one of the most widely studied proteins; its structure is well known and its antigenic characteristics have been described in several papers. The aim of this research was the identification of the BSA antigenic determinants.METHODS:
This study was performed using limited proteolysis and an immunoblotting technique, in which a commercial murine antibody and sera from children sensitized to BSA were used.RESULTS:
Findings suggest amino acids (aa) 524-598 as an epitopic area for human species. The most critical sequence seems to be aa 524-542, even if it must be included in a longer fragment to be recognized by antibodies. Murine IgG antibodies also recognize fragments contained in the first half (NH(2)-terminal portion) of BSA.CONCLUSIONS:
The results presented in this study indicate that the epitopic sites of an antigenic protein can be different when different species are considered, so that data obtained with antibodies from animal species cannot be directly extrapolated to the behavior of human IgEs.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serum Albumin, Bovine
/
Food Hypersensitivity
/
Meat
/
Epitopes
Type of study:
Prognostic_studies
Limits:
Animals
/
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Language:
En
Journal:
Int Arch Allergy Immunol
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2001
Document type:
Article
Affiliation country:
Italy
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND