No evidence of oxidant events in amphotericin B cytotoxicity versus L. infantum promastigotes.
Parasite
; 8(4): 335-41, 2001 Dec.
Article
in En
| MEDLINE
| ID: mdl-11802270
ABSTRACT
Amphotericin B is used for the treatment of systemic mycoses and visceral leishmaniasis. The objective of our study was to evaluate the impact of catalase, ascorbic acid and ketoconazole on the amphotericin B toxicity towards Leishmania promastigotes membrane by two flow cytometric tests, the membrane potential assay using a cationic dye, [DiOC5(3)], and the membrane permeability test using propidium iodide. The collapse of membrane potential appeared at amphotericin B concentrations weaker than those assessed by the membrane permeability test. The binding of amphotericin B to membrane sterol was not modified by catalase or ascorbic acid whereas amphotericin B-induced growth inhibition could be modulated by these products. The permeabilizing effect of amphotericin B on parasite membrane was strongly reduced in the presence of ketoconazole. These results confirmed the pore hypothesis of amphotericin B action and suggested that flow cytometric methods constituted a valuable alternative to conventional methods for assessing the effect of drugs on cellular membrane and evaluating parasite susceptibility to polyene antibiotics.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Amphotericin B
/
Leishmania infantum
/
Leishmaniasis, Visceral
/
Mycoses
/
Antiprotozoal Agents
Limits:
Animals
Language:
En
Journal:
Parasite
Journal subject:
PARASITOLOGIA
Year:
2001
Document type:
Article
Affiliation country:
France