High-efficiency solid-phase capture using glass beads bonded to microcentrifuge tubes: immunoprecipitation of proteins from cell extracts and assessment of ras activation.

Chen, Jeffrey C; von Lintig, Friederike C; Jones, Stephen B; Huvar, Ivana; Boss, Gerry R

Chen, Jeffrey C; von Lintig, Friederike C; Jones, Stephen B; Huvar, Ivana; Boss, Gerry R.

Affiliation

Chen JC; Department of Medicine and Cancer Center, University of California at San Diego, La Jolla, California 92093-0652, USA.

Anal Biochem ; 302(2): 298-304, 2002 Mar 15.

Article in En | MEDLINE | ID: mdl-11878811

Subject(s)

Cell Extracts/chemistry; Dimethyl Suberimidate/chemistry; Guanine Nucleotide Dissociation Inhibitors/analysis; Staphylococcal Protein A/chemistry; ras Proteins/analysis; 3T3 Cells/chemistry; Animals; Guanine Nucleotide Dissociation Inhibitors/immunology; HL-60 Cells/chemistry; Humans; Mice; Microspheres; Polypropylenes/chemistry; Precipitin Tests/methods; Proteins/analysis; Proteins/immunology; Silanes/chemistry; Tumor Cells, Cultured; ras Proteins/immunology; rho-Specific Guanine Nucleotide Dissociation Inhibitors

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Collection: 01-internacional Database: MEDLINE Main subject: Staphylococcal Protein A / Cell Extracts / Ras Proteins / Guanine Nucleotide Dissociation Inhibitors / Dimethyl Suberimidate Limits: Animals / Humans Language: En Journal: Anal Biochem Year: 2002 Document type: Article Affiliation country: United States Country of publication: United States

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