A dual reporter screening system identifies the amino acid at position 82 in Flp site-specific recombinase as a determinant for target specificity.

Voziyanov, Yuri; Stewart, A Francis; Jayaram, Makkuni

Voziyanov, Yuri; Stewart, A Francis; Jayaram, Makkuni.

Affiliation

Voziyanov Y; Section of Molecular Genetics and Microbiology, University of Texas, Austin, TX 78712-1095, USA. voziyanov@mail.utexas.edu

Nucleic Acids Res ; 30(7): 1656-63, 2002 Apr 01.

Article in En | MEDLINE | ID: mdl-11917027

Subject(s)

Amino Acids/genetics; DNA Nucleotidyltransferases/genetics; Recombination, Genetic/genetics; Amino Acid Substitution; Base Sequence; Binding Sites/genetics; DNA Nucleotidyltransferases/metabolism; Escherichia coli/genetics; Genes, Reporter/genetics; Lac Operon/genetics; Mutation; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Substrate Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombination, Genetic / DNA Nucleotidyltransferases / Amino Acids Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Nucleic Acids Res Year: 2002 Document type: Article Affiliation country: United States Country of publication: United kingdom

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