Measurement of free and membrane-bound cathepsin G in human neutrophils using new sensitive fluorogenic substrates.

Attucci, Sylvie; Korkmaz, Brice; Juliano, Luiz; Hazouard, Eric; Girardin, Catherine; Brillard-Bourdet, Michèle; Réhault, Sophie; Anthonioz, Philippe; Gauthier, Francis

Attucci, Sylvie; Korkmaz, Brice; Juliano, Luiz; Hazouard, Eric; Girardin, Catherine; Brillard-Bourdet, Michèle; Réhault, Sophie; Anthonioz, Philippe; Gauthier, Francis.

Affiliation

Attucci S; INSERM EMI-U 00-10 Protéases et Vectorisation, Laboratory of Enzymology and Protein Chemistry, University François Rabelais, 2bis Bd Tonnellé, 37032 TOURS Cedex, France.

Biochem J ; 366(Pt 3): 965-70, 2002 Sep 15.

Article in En | MEDLINE | ID: mdl-12088507

Subject(s)

Cathepsins/chemistry; Fluorescent Dyes/pharmacology; Neutrophils/metabolism; Binding Sites; Cathepsin G; Cathepsins/metabolism; Chromatography; Dinitrobenzenes/pharmacology; Humans; Hydrolysis; Kinetics; Oligopeptides/pharmacology; Serine Endopeptidases; Spectrometry, Fluorescence/methods

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cathepsins / Fluorescent Dyes / Neutrophils Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biochem J Year: 2002 Document type: Article Affiliation country: France

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