Multiplexing of canine microsatellite markers for whole-genome screens.

ABSTRACT

A set of 172 canine microsatellite markers, termed minimal screening set 1 (MSS1), was recently characterized for use in whole-genome screens. We report here the multiplexing of 155 MSS1 markers into 48 multiplex sets. Amplification of the multiplex sets is achieved using a single thermal cycling program. The markers are labeled with fluorescent dyes and optimized for resolution on an ABI 310 Genetic Analyzer or ABI 377 Sequencer. The multiplexing strategy involves amplifying combinations of markers so that no two markers with the same dye and product size overlap. Multiplexing the MSS1 provides an efficient tool for the collection of genotypes and streamlines whole-genome screens. Screening the canine genome for linkage of markers with various hereditary diseases facilitates identification of affected and carrier individuals, thereby providing researchers and clinicians with an additional diagnostic tool.