Evaluating biofilm growth of two oral pathogens.
Lett Appl Microbiol
; 35(6): 552-6, 2002.
Article
in En
| MEDLINE
| ID: mdl-12460443
ABSTRACT
AIMS:
To determine the expediency of a microtitre assay system for establishing, quantifying and antimicrobial testing of two representative oral pathogens. METHODS ANDRESULTS:
Streptococcus mutans and Porphyromonas gingivalis were used. Morphological characteristics of the attached population were evaluated. Biofilm growth was evaluated spectrophotometrically (undisturbed and 1 N NaOH dissipated biofilm). The minimum concentration of chlorhexidine gluconate that inhibited biofilm growth was determined. Growth of the biofilms was successfully monitored by direct optical density measurements or those re-suspended in 1 N NaOH. The latter was necessary when glucans were present in Strep. mutans biofilms. The minimum concentration of chlorhexidine gluconate that inhibited biofilm growth was 1.25 microg ml(-1) for both species. The attached bacteria exhibited common biofilm characteristics. SIGNIFICANCE AND IMPACT OF THE STUDY The assay system developed was especially useful for monitoring the growth of adherent Strep. mutans in the presence of glucans, which is particularly significant for the study of anti-plaque chemicals.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptococcus mutans
/
Porphyromonas gingivalis
/
Biofilms
Type of study:
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
Lett Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2002
Document type:
Article
Affiliation country:
United States