Identification of neuropeptide FF-related peptides in human cerebrospinal fluid by mass spectrometry.
FEBS Lett
; 532(3): 313-8, 2002 Dec 18.
Article
in En
| MEDLINE
| ID: mdl-12482585
ABSTRACT
Several neuropeptide FF (NPFF)-related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse-phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co-migrating with NPFF-related synthetic peptides, were characterized by capillary HPLC coupled on-line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro-NPFF(A) precursor, NPAF (AGEGLNSQFWSLAAPQRF-NH2) and NPSF (SLAAPQRF-NH2), were identified. The monitoring of NPFF-related peptides in human CSF can be helpful to understand their roles in pain sensitivity.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligopeptides
/
Peptides
/
Mass Spectrometry
/
Cerebrospinal Fluid
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2002
Document type:
Article
Affiliation country:
France