Single-tube nested PCR using immobilized internal primers.

Abath, F G C; Melo, F L; Werkhauser, R P; Montenegro, L; Montenegro, R; Schindler, H C

Abath, F G C; Melo, F L; Werkhauser, R P; Montenegro, L; Montenegro, R; Schindler, H C.

Affiliation

Abath FG; Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Av. Moraes Rego s/n. Cidade Universitária, 50670-420, Recife, Brazil. fabath@cpqam.fiocruz.br

Biotechniques ; 33(6): 1210-2, 1214, 2002 Dec.

Article in En | MEDLINE | ID: mdl-12503300

Subject(s)

DNA Primers/chemistry; Polymerase Chain Reaction/methods; Animals; Biomphalaria/genetics; DNA, Protozoan/genetics; Desiccation; Equipment Contamination/prevention & control; Humans; Mice; Plasmodium/genetics; Polymerase Chain Reaction/instrumentation; Reproducibility of Results; Schistosoma mansoni/genetics; Schistosomiasis mansoni/diagnosis; Solubility; Templates, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction / DNA Primers Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals / Humans Language: En Journal: Biotechniques Year: 2002 Document type: Article Affiliation country: Brazil

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