[Hairy root induction and in vitro cultivation of Pueraria lobata willa. ohwi].
Zhongguo Zhong Yao Za Zhi
; 25(9): 525-7, 535, 2000 Sep.
Article
in Zh
| MEDLINE
| ID: mdl-12516461
ABSTRACT
OBJECTIVE:
To study the hairy root induction and hormone-free in vitro liquid cultivation of Pueraria lobata (Willd) Ohwi.METHOD:
Co-cultivation of super-virulent Agrobacterium rhizogenes R1601 with P. lobata leaves in vitro.RESULTS:
Hairy roots of rapid growth, high branches and plagiotropism developed vigorously on the surface of leaves, exhibiting rapid growth and resistance to kanamycin in hormone-free medium in vitro.CONCLUSION:
A method of hairy root induction with A. rhizogenes as well as a system hairy root liquid cultivation in vitro for P. lobata have been established.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Medicinal
/
Rhizobium
/
Pueraria
Language:
Zh
Journal:
Zhongguo Zhong Yao Za Zhi
Journal subject:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Year:
2000
Document type:
Article
Affiliation country:
China