Calcium-Calmodulin-Dependent Protein Kinases/metabolism; Endothelium, Vascular/metabolism; Endothelium, Vascular/physiology; Muscle, Smooth, Vascular/physiology; Nitric Oxide/biosynthesis; Acetylcholine/pharmacology; Animals; Aorta, Thoracic/drug effects; Benzylamines/pharmacology; Calcimycin/pharmacology; Calcium/pharmacology; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors; Cell Line; Cells, Cultured; Electric Stimulation; Endothelium, Vascular/enzymology; Enzyme Inhibitors/pharmacology; Immunoblotting; In Vitro Techniques; Muscle Contraction/physiology; Muscle Relaxation/physiology; Muscle, Smooth, Vascular/enzymology; Nitric Oxide Donors/pharmacology; Nitric Oxide Synthase/metabolism; Nitric Oxide Synthase Type III; Phosphorylation; Rats; S-Nitroso-N-Acetylpenicillamine/pharmacology; Sulfonamides/pharmacology; Swine; Thapsigargin/pharmacology; Vasodilator Agents/pharmacology