Measuring gluconeogenesis using a low dose of 2H2O: advantage of isotope fractionation during gas chromatography.
Am J Physiol Endocrinol Metab
; 284(5): E1043-8, 2003 May.
Article
in En
| MEDLINE
| ID: mdl-12582010
ABSTRACT
The contribution of gluconeogenesis to glucose production can be measured by enriching body water with (2)H(2)O to approximately 0.5% (2)H and determining the ratio of (2)H that is bound to carbon-5 vs. carbon-2 of blood glucose. This labeling ratio can be measured using gas chromatography-mass spectrometry after the corresponding glucose carbons are converted to formaldehyde and then to hexamethylenetetramine (HMT). We present a technique for integrating ion chromatograms that allows one to use only 0.05% (2)H in body water (i.e., 10 times less than the current dose). This technique takes advantage of the difference in gas chromatographic retention times of naturally labeled HMT and [(2)H]HMT. We discuss the advantage(s) of using a low dose of (2)H(2)O to quantify the contribution of gluconeogenesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Deuterium Oxide
/
Gluconeogenesis
Limits:
Animals
Language:
En
Journal:
Am J Physiol Endocrinol Metab
Journal subject:
ENDOCRINOLOGIA
/
FISIOLOGIA
/
METABOLISMO
Year:
2003
Document type:
Article
Affiliation country:
United States