Towards the identification of cassava root protein genes.

De Souza, C R B; Carvalho, L J C B; De Almeida, E R P; Gander, E S

De Souza, C R B; Carvalho, L J C B; De Almeida, E R P; Gander, E S.

Affiliation

De Souza CR; Embrapa-Recursos Genéticos e Biotecnologia-CENARGEN, Parque Estação Biológica, PqEB-Final Av. W5 Norte, 70770-900 Brasília-DF, Brazil.

Plant Foods Hum Nutr ; 57(3-4): 353-63, 2002.

Article in En | MEDLINE | ID: mdl-12602941

ABSTRACT

ABSTRACT

The protein population of cassava root layers was characterized by SDS-PAGE and bidimensional polyacrylamide gel electrophoresis. SDS-Page revealed the presence of a protein population in the molecular weight range between 94 and 20 kDa. The expression pattern of these proteins was well-defined within the different layers. Partial protein sequence analyses and preliminary results on the layer-specific expression pattern obtained with Northern analyses are presented.

Subject(s)

Manihot/genetics; Plant Proteins/genetics; Amino Acid Sequence; Base Sequence; Blotting, Northern; DNA, Plant/chemistry; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Gene Amplification; Manihot/chemistry; Molecular Sequence Data; Molecular Weight; Plant Proteins/chemistry; Plant Proteins/isolation & purification; Plant Roots/chemistry; Plant Roots/genetics; Plants, Genetically Modified; Polymerase Chain Reaction; Prolamins; RNA, Plant/analysis; Sequence Analysis, DNA

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Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Manihot Type of study: Diagnostic_studies Language: En Journal: Plant Foods Hum Nutr Journal subject: BOTANICA / CIENCIAS DA NUTRICAO Year: 2002 Document type: Article Affiliation country: Brazil

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