Effect of zinc on bone metabolism in fetal mouse limb culture.
Biomed Environ Sci
; 15(4): 323-9, 2002 Dec.
Article
in En
| MEDLINE
| ID: mdl-12642989
ABSTRACT
OBJECTIVE:
To determine the effects of zinc-deficiency and zinc-excess on bone metabolism.METHODS:
We developed the culture model of fetal mouse limbs (16th day) cultivated in self-made rotator with continuing flow of mixed gas for six days in vitro. The cultured limbs were examined by the techniques of 45Ca tracer and X-roentgenography.RESULTS:
The right limbs cultivated had longer bone length, higher bone density than the left limbs uncultivated from the same embryo; and histologically, the right limbs had active bone cell differentiation, proliferation, increased bone trabecula, clearly calcified cartilage matrix, and osteogenic tissue. Compared with the control group, the zinc-deficient group and zinc-excess (Zn2+ 120 mumol/L) group contained less osteocalcin (BGP) and 45Ca content, and lower AKP activity; whereas zinc-normal (Zn2+ 45 mumol/L and Zn2+ 70 mumol/L) groups contained more BGP and 45Ca contents, and higher AKP (alkaline phosphatase) activity.CONCLUSION:
Both zinc-deficiency and zinc-excess can alter bone growth and normal metabolism. The results indicate that the culture model of fetal mouse limbs (16th day) in vitro can be used as a research model of bone growth and development.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Zinc
/
Bone and Bones
/
Embryonic and Fetal Development
Type of study:
Prognostic_studies
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Biomed Environ Sci
Journal subject:
SAUDE AMBIENTAL
Year:
2002
Document type:
Article
Affiliation country:
China