A survey for BVDV antibodies in cattle farms in Slovakia and genetic typing of BVDV isolates from imported animals.
Acta Vet Hung
; 51(2): 229-36, 2003.
Article
in En
| MEDLINE
| ID: mdl-12737050
A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5'-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1 h group.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bovine Virus Diarrhea-Mucosal Disease
/
Diarrhea Viruses, Bovine Viral
/
Antibodies, Viral
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Acta Vet Hung
Year:
2003
Document type:
Article
Affiliation country:
Slovakia
Country of publication:
Hungary