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A survey for BVDV antibodies in cattle farms in Slovakia and genetic typing of BVDV isolates from imported animals.
Vilcek, S; Mojzisová, Jana; Bajová, Viera; Paulík, S; Strojný, L; Durkovic, B; Hipíková, Vlasta.
Affiliation
  • Vilcek S; Department of Parasitology and Infectious Diseases, University of Veterinary Medicine, Komenského 73, 041 81 Kosice, Slovakia. vilcek@uvm.sk
Acta Vet Hung ; 51(2): 229-36, 2003.
Article in En | MEDLINE | ID: mdl-12737050
A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5'-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1 h group.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Bovine Virus Diarrhea-Mucosal Disease / Diarrhea Viruses, Bovine Viral / Antibodies, Viral Limits: Animals Country/Region as subject: Europa Language: En Journal: Acta Vet Hung Year: 2003 Document type: Article Affiliation country: Slovakia Country of publication: Hungary
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Collection: 01-internacional Database: MEDLINE Main subject: Bovine Virus Diarrhea-Mucosal Disease / Diarrhea Viruses, Bovine Viral / Antibodies, Viral Limits: Animals Country/Region as subject: Europa Language: En Journal: Acta Vet Hung Year: 2003 Document type: Article Affiliation country: Slovakia Country of publication: Hungary