Cloning expression and characterization of a thermostable exopolygalacturonase from Thermotoga maritima.
Carbohydr Res
; 338(12): 1333-7, 2003 Jun 16.
Article
in En
| MEDLINE
| ID: mdl-12791288
ABSTRACT
A gene encoding for a thermostable exopolygalacturonase (exo-PG) from hyperthermophilic Thermotoga maritima has been cloned into a T7 expression vector and expressed in Escherichia coli. The gene encoded a polypeptide of 454 residues with a molecular mass of 51,304 Da. The recombinant enzyme was purified to homogeneity by heat treatment and nickel affinity chromatography. The thermostable enzyme had maximum of hydrolytic activity for polygalacturonate at 95 degrees C, pH 6.0 and retains 90% of activity after heating at 90 degrees C for 5 h. Study of the catalytic activity of the exopolygalacturonase, investigated by means of 1H NMR spectroscopy revealed an inversion of configuration during hydrolysis of alpha-(1-->4)-galacturonic linkage.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thermotoga maritima
/
Glycoside Hydrolases
Language:
En
Journal:
Carbohydr Res
Year:
2003
Document type:
Article
Affiliation country:
France