Solid phase microextraction-gas chromatography for quantifying headspace hexanal above freeze-dried chicken myofibrils.
J Agric Food Chem
; 51(15): 4185-90, 2003 Jul 16.
Article
in En
| MEDLINE
| ID: mdl-12848482
ABSTRACT
A method using solid phase microextraction (SPME) combined with gas chromatography/mass spectrometry (GC/MS) was developed and used to determine the oxidation of freeze-dried chicken myofibrils spiked with methyl linoleate. Freeze-dried chicken myofibrils were found to act as a significant reservoir for hexanal. Recovery of hexanal emissions from the headspace above spiked myofibrils was 95% using a 5 min sampling time, with a total analysis time of approximately 12 min/sample. The SPME-GC/MS working linear response was from 0.01 to 10 mg hexanal/L (r( 2) = 0.995). Freeze-dried chicken myofibrils with added methyl linoleate (0.6 mmol/g of protein) were stored at 50 degrees C at water activities of 0.30 and 0.75 for 0, 12, 27, and 50 h. Lipid oxidation was determined using SPME-GC/MS to measure headspace hexanal concentration, the thiobarbituric acid reactive substances assay (TBARS) to quantify malonaldehyde, and a conjugated diene assay. Lipid oxidation was influenced by storage time and water activity. A strong correlation (r = 0.938) existed between SPME-GC/MS and TBARS. The use of SPME-GC/MS was a sensitive and rapid method for detecting hexanal as an indicator of lipid oxidation in chicken myofibrils.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lipid Peroxidation
/
Chickens
/
Chromatography, Gas
/
Aldehydes
/
Myofibrils
Limits:
Animals
Language:
En
Journal:
J Agric Food Chem
Year:
2003
Document type:
Article
Affiliation country:
United States