[The effects of three plastic additives on the proliferation of MCF-7 cell].
Zhonghua Yu Fang Yi Xue Za Zhi
; 37(3): 150-3, 2003 May.
Article
in Zh
| MEDLINE
| ID: mdl-12880557
ABSTRACT
OBJECTIVE:
To explore the effect of environmental estrogens on the proliferation of breast cancer cell line MCF-7.METHODS:
The tested compounds were n-4-nonyphenol, Bisphenol A and dibutylphthalate. Human estradiol-dependent MCF-7 breast cancer cells were grown in DMEM medium containing 10% bovine serum. Five days before the addition of the test compounds, the cells were washed by phosphate-buffered saline, and the medium was substituted with a phenol red-free DMEM medium containing 5% dextral charcoal-stripped FBS. The respective test compound was added in fresh medium and the control cell received only the vehicle (ethanol). The proliferation of MCF-7 was analyzed by the MTT assay, (3)H-TdR incorporation assay and flow cytometry.RESULTS:
Compared with the ethanol control, the proliferation and DNA synthesis of the test cells treated with n-4-nonyphenol (8 x 10(-7) mol/L, 96 h), Bisphenol A (8 x 10(-7) mol/L, 96 h) or dibutylphthalate (32 x 10(-6) mol/L, 96 h) treatment was markedly enhanced in a time-dependent and dose-dependent manner.CONCLUSION:
n-4-Nonyphenol, Bisphenol A and dibutylphthalate enhanced the proliferation of human breast cancer cell in vitro, which may demonstrate an estrogenic activity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Environmental Pollutants
/
Estrogens, Non-Steroidal
Limits:
Female
/
Humans
Language:
Zh
Journal:
Zhonghua Yu Fang Yi Xue Za Zhi
Year:
2003
Document type:
Article
Affiliation country:
China