Theoretical modeling of prion disease incubation.
Biophys J
; 85(2): 707-18, 2003 Aug.
Article
in En
| MEDLINE
| ID: mdl-12885622
ABSTRACT
We apply a theoretical aggregation model to laboratory and epidemiological prion disease incubation time data. In our model, slow growth of misfolded protein aggregates from small initial seeds controls the latent or lag phase; aggregate fissioning and subsequent spreading leads to an exponential growth phase. Our model accounts for the striking reproducibility of incubation times for high dose inoculation of lab animals. In particular, low dose yields broad incubation time distributions, and increasing dose narrows distributions and yields sharply defined onset times. We also explore how incubation time statistics depend upon aggregate morphology. We apply our model to fit the experimental dose-incubation curves for distinct strains of scrapie, and explain logarithmic variation at high dose and deviations from logarithmic behavior at low dose. We use this to make testable predictions for infectivity time-course experiments.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prion Diseases
/
PrPSc Proteins
/
Models, Biological
/
Neurons
Type of study:
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Biophys J
Year:
2003
Document type:
Article
Affiliation country:
United States