Effects of neutral, cationic, and anionic chromium ascorbate complexes on isolated human mitochondrial and genomic DNA.
J Biochem Mol Biol
; 36(4): 403-8, 2003 Jul 31.
Article
in En
| MEDLINE
| ID: mdl-12895300
ABSTRACT
The relative activities of neutral, cationic, and anionic chromium ascorbate complexes toward isolated human mitochondrial and genomic DNA were investigated at physiologically relevant conditions by agarose gel electrophoresis. A direct relationship between the charge of the Cr(III) species and their DNA-damaging properties was found. The cationic species were found to be fully capable of DNA-cleavage, even in short incubation periods. Incubations were also performed in the presence of amino acids. No apparent effect was observed under the applied experimental conditions to facilitate or prevent damage through the ternary amino acid-Cr-DNA adduct formation or binary chromium-amino acid complex formation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ascorbic Acid
/
DNA Damage
/
DNA
/
DNA, Mitochondrial
/
Chromium
/
Chromium Compounds
Limits:
Humans
Language:
En
Journal:
J Biochem Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
2003
Document type:
Article
Affiliation country:
Turkey