An alternative to PVP for slowing sperm prior to ICSI.
Hum Reprod
; 18(9): 1887-9, 2003 Sep.
Article
in En
| MEDLINE
| ID: mdl-12923144
ABSTRACT
BACKGROUND:
There is a growing awareness of potential problems in exposing sperm to polyvinylpyrrolidone (PVP) to slow their motility, a procedure commonly used prior to ICSI. The study presented here evaluates an alternative product for slowing sperm motility, which contains hyaluronate, a substance found naturally in the reproductive tract.METHODS:
Computerized sperm motility analysis was used to compare the motilities of sperm exposed to either a PVP-containing product (ICSI-100), or a hyaluronate-containing product (SpermCatch), or control sperm resuspended in a sperm maintenance medium. A subjective assessment was made of the ease with which sperm could be isolated and be drawn into, and expelled from, an injection pipette after having their tails nicked. Sperm exposed to either ICSI-100 or SpermCatch were used for ICSI. Fertilization rate, zygote development, grading, and outcome of transfer were recorded for the two treatment groups.RESULTS:
The hyaluronate-containing product slowed sperm motility sufficiently for the sperm to be captured in an injection pipette, was easy to draw into and expel from the pipette, prevented sperm sticking to plastic or glassware, and did not affect post-injection zygote development. Clinical pregnancy rates were similar for the two groups.CONCLUSIONS:
This product represents an alternative to PVP for slowing sperm motility prior to ICSI.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sperm Motility
/
Tissue and Organ Harvesting
/
Sperm Injections, Intracytoplasmic
/
Hyaluronic Acid
Type of study:
Clinical_trials
Limits:
Female
/
Humans
/
Male
/
Pregnancy
Language:
En
Journal:
Hum Reprod
Journal subject:
MEDICINA REPRODUTIVA
Year:
2003
Document type:
Article
Affiliation country:
Turkey
Country of publication:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM