Isolation of a peptide inhibitor of human rhinovirus.
Virology
; 313(1): 170-83, 2003 Aug 15.
Article
in En
| MEDLINE
| ID: mdl-12951031
ABSTRACT
Cell culture-based transdominant genetic techniques provide new methods for discovering peptide/RNA modulators of cellular pathways. We applied this technology to isolate a peptide inhibitor of human rhinovirus. A green fluorescent protein (GFP)-scaffolded library of cDNA fragments was expressed in HeLa cells from a retroviral vector and screened for inhibitors of rhinovirus-mediated cell killing. A DNA clone, I421, increased cell survival in an HRV14 challenge assay from less than 0.5% to greater than 60%. It encodes a 53-amino-acid C-terminal extension of the GFP scaffold. Particular subclones of Hela cells expressing I421 (exemplified by I421dp3) show a delay in virus production and a 50-fold decrease in viral RNA levels at 6-8 h postinfection. HRV2, HRV14, and HRV16 show a dramatic decrease in plaque-forming ability on I421dp3 while Coxsackievirus B3 showed a small reduction. Levels of ICAM-1, the receptor for the main rhinovirus serotype, are not altered in I421dp3.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antiviral Agents
/
Peptides
/
Rhinovirus
Limits:
Humans
Language:
En
Journal:
Virology
Year:
2003
Document type:
Article
Affiliation country:
United States