[Determination of deoxyribonucleic acid with alkali blue 6B by resonance light-scattering method].
Guang Pu Xue Yu Guang Pu Fen Xi
; 23(3): 458-60, 2003 Jun.
Article
in Zh
| MEDLINE
| ID: mdl-12953513
ABSTRACT
Characteristics of the interaction between alkali blue 6B with deoxyribonucleic acid (DNA) was studied by resonance light-scattering (RLS) method in the pH 2.90 medium. The effects of interference substances and the optimization of procedure were investigated, and a new method based on RLS was established. The method can be used to determine the DNA at nanogram level. The linear range of the DNA is 10-100.4 micrograms.L-1 with a correlation coefficient of 0.9913. The detection limit is 4.2 micrograms-L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory results.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzenesulfonates
/
DNA
/
Aniline Compounds
Language:
Zh
Journal:
Guang Pu Xue Yu Guang Pu Fen Xi
Year:
2003
Document type:
Article
Affiliation country:
China