Conversion of Bfl-1, an anti-apoptotic Bcl-2 family protein, to a potent pro-apoptotic protein by fusion with green fluorescent protein (GFP).
FEBS Lett
; 551(1-3): 29-36, 2003 Sep 11.
Article
in En
| MEDLINE
| ID: mdl-12965200
ABSTRACT
Human Bfl-1 is an anti-apoptotic Bcl-2 family member. Here, we found that Bfl-1 was converted into a potent death-promoting protein by green fluorescent protein (GFP) fusion with its N-terminus. The transient expression of GFP-Bfl-1 induced cytochrome c release and triggered apoptosis in 293T cells, which depended on the mitochondrial localization of GFP-Bfl-1. Apoptosis induced by GFP-Bfl-1 was significantly blocked by the pan-caspase inhibitor carbobenzoxy-Val-Ala-Asp-fluoromethyl ketone, but was not blocked by either Bcl-xL or Bfl-1. Our findings provide a useful model for understanding the structural basis of Bcl-2 family proteins that act in an opposite way despite sharing structural similarity between anti-apoptotic and pro-apoptotic proteins.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2003
Document type:
Article
Affiliation country:
South Korea