Alzheimer skin fibroblasts show increased susceptibility to free radicals.

Tesco, G; Latorraca, S; Piersanti, P; Piacentini, S; Amaducci, L; Sorbi, S

Tesco, G; Latorraca, S; Piersanti, P; Piacentini, S; Amaducci, L; Sorbi, S.

Affiliation

Tesco G; Department of Neurological and Psychiatric Sciences, University of Florence, Italy.

Mech Ageing Dev ; 66(2): 117-20, 1992 Nov.

Article in En | MEDLINE | ID: mdl-1365838

ABSTRACT

ABSTRACT

We have studied the response to toxic oxygen metabolites of fibroblasts derived from skin biopsies of 5 patients with familial (FAD) and 4 with sporadic (AD) Alzheimer's disease compared with those derived from 4 normal controls. Fibroblasts were damaged by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde by 50 munits of xanthine-oxidase (Xo). To quantify cell damage we measured lactate dehydrogenase (LDH) activity in the culture medium and cell viability in fibroblast cultures. We found a significant increase in LDH activity in the FAD vs. controls and also in the AD vs. controls.

Subject(s)

Alzheimer Disease/metabolism; Reactive Oxygen Species/metabolism; Skin/metabolism; Acetaldehyde/metabolism; Aging/metabolism; Alzheimer Disease/genetics; Cell Line; Cell Survival; Fibroblasts/metabolism; Free Radical Scavengers; Free Radicals; Humans; L-Lactate Dehydrogenase/metabolism; Superoxide Dismutase/metabolism; Xanthine Oxidase/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Skin / Reactive Oxygen Species / Alzheimer Disease Limits: Humans Language: En Journal: Mech Ageing Dev Year: 1992 Document type: Article Affiliation country: Italy Country of publication: IE / IRELAND / IRLANDA

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