Bacteriophage cloning and Escherichia coli expression of a human IgM Fab.

Hay, B N; Sorge, J A; Shopes, B

Hay, B N; Sorge, J A; Shopes, B.

Affiliation

Hay BN; Stratacyte Corporation, La Jolla, CA 92037.

Hum Antibodies Hybridomas ; 3(2): 81-5, 1992 Apr.

Article in En | MEDLINE | ID: mdl-1385989

ABSTRACT

ABSTRACT

We have combined the molecular biology methods of the polymerase chain reaction and recombinant DNA cloning in bacteriophage lambda to express a human IgM Fab in Escherichia coli using genes derived from an Epstein-Barr virus transformed cell line. This method comprises three cDNA amplifications and a single cloning step, culminating in the stable overexpression of mammalian heterodimeric recombinant protein in a prokaryotic host.

Subject(s)

Genes, Immunoglobulin; Immunoglobulin Fab Fragments/genetics; Immunoglobulin M/genetics; Bacteriophage lambda/genetics; Base Sequence; Cloning, Molecular; DNA/genetics; Escherichia coli/genetics; Gene Expression; Humans; Hybridomas/immunology; Molecular Sequence Data; Polymerase Chain Reaction/methods

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Collection: 01-internacional Database: MEDLINE Main subject: Immunoglobulin M / Immunoglobulin Fab Fragments / Genes, Immunoglobulin Limits: Humans Language: En Journal: Hum Antibodies Hybridomas Journal subject: ALERGIA E IMUNOLOGIA Year: 1992 Document type: Article

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