Adjuvants recognized by toll-like receptors inhibit the induction of polarized type 2 T cell responses by natural attachment (G) protein of respiratory syncytial virus.
Vaccine
; 21(27-30): 4348-58, 2003 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-14505917
ABSTRACT
Immunization with native fusion (F) protein from respiratory syncytial virus (RSV) adsorbed to alum adjuvant generates greater than fourfold rises in serum neutralizing antibody titers in approximately 50% of seropositive humans. Using BALB/c mice we demonstrate herein that enhanced neutralization titers and accelerated clearance of virus from the lungs after challenge are possible if the attachment (G) glycoprotein is added to F protein-based vaccines. We further reveal for the first time that polarized type 2 T cell responses and immunopathology associated with G protein are inhibited by adjuvants recognized by toll-like receptors (TLR). Co-formulation with compounds that targeted TLR-2, TLR-3, TLR-4, or TLR-9 elicited significantly diminished type 2 T cell responses that caused granulocytic inflammation and eosinophilia in the airways after challenge. These results were not observed with recombinant IL-12 or QS-21. The data are important for improving combination vaccines for RSV.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Membrane Glycoproteins
/
T-Lymphocytes
/
Viral Envelope Proteins
/
Adjuvants, Immunologic
/
Respiratory Syncytial Virus, Human
/
Receptors, Cell Surface
Limits:
Animals
Language:
En
Journal:
Vaccine
Year:
2003
Document type:
Article
Affiliation country:
United States