Isolation of anticoagulant from the venom of tick, Boophilus calcaratus, from Uzbekistan.
Thromb Res
; 110(4): 235-41, 2003 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-14512088
ABSTRACT
Boophilus calcaratus is a tick found in Central Asia and a common parasite to domestic animals. Venom from this tick was fractionated by two-step column chromatography, Sephadex G-75, and DEAE-Sephadex A-25. The homogeneity of the anticoagulant was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified component is named calcaratin and has a molecular weight of 14,500. The effect of the purified anticoagulant component (calcaratin) on various sites of the blood coagulation cascade scheme was examined and compared with crude venom. The chromogenic substrates S-2238 (H-D-Phe-Pip-Arg-pNA 2HCl) for thrombin and S-2765 (N-alpha-Z-D-Arg-Gly-Arg-pNA 2HCl) for factor Xa were also investigated. Activated partial clotting times were all prolonged, suggesting the anticoagulation nature of the purified component and crude venom. Prolongation of fibrinogen clotting time (FCT) is highly suggestive of the antithrombin property of the purified component and its original venom.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arthropod Venoms
/
Ticks
/
Anticoagulants
Limits:
Animals
Country/Region as subject:
Asia
/
Europa
Language:
En
Journal:
Thromb Res
Year:
2003
Document type:
Article
Affiliation country:
United States
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA