Enhanced in vivo delivery of antisense oligonucleotides to restore dystrophin expression in adult mdx mouse muscle.
FEBS Lett
; 552(2-3): 145-9, 2003 Sep 25.
Article
in En
| MEDLINE
| ID: mdl-14527677
ABSTRACT
The use of antisense oligonucleotides (AOs) to induce exon skipping leading to generation of an in-frame dystrophin protein product could be of benefit in around 70% of Duchenne muscular dystrophy patients. We describe the use of hyaluronidase enhanced electrotransfer to deliver uncomplexed 2'-O-methyl modified phosphorothioate AO to adult dystrophic mouse muscle, resulting in dystrophin expression in 20-30% of fibres in tibialis anterior muscle after a single injection. Although expression was transient, many of the corrected fibres initially showed levels of dystrophin expression well above the 20% of endogenous previously shown to be necessary for phenotypic correction of the dystrophic phenotype.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dystrophin
/
Oligodeoxyribonucleotides, Antisense
/
Muscular Dystrophy, Animal
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
FEBS Lett
Year:
2003
Document type:
Article
Affiliation country:
United kingdom