Detection of N-myc gene amplification in bone marrow specimen of stage IV neuroblastoma patients.
Cancer Detect Prev
; 16(3): 185-92, 1992.
Article
in En
| MEDLINE
| ID: mdl-1458508
ABSTRACT
N-myc gene amplification is an unfavorable prognostic factor in neuroblastoma; therefore, its detection might have therapeutical consequences. Because of the reliability of noninvasive diagnostic methods such as X-ray examination and measurement of vanilymandelic acid and the lack of technical unavailability at most institutions, the determination of the N-myc status of neuroblastoma often is not done. In our investigation of 14 neuroblastoma patients, we could demonstrate N-myc gene amplification in the bone marrow of 5 patients with neuroblastoma stage IV disease and in bone marrow infiltration without enrichment of neuroblastoma cells. Otherwise, no information about the tumor content of N-myc gene copies at the time of initial diagnosis could be obtained. At the subsequent resection, the N-myc gene amplification was confirmed by the additional Southern blot analysis and in situ hybridization of tumor tissue. Furthermore, the N-myc status of bone marrow was analyzed during the stages of chemotherapy in three cases.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bone Marrow
/
Gene Amplification
/
Genes, myc
/
Neuroblastoma
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Cancer Detect Prev
Year:
1992
Document type:
Article
Affiliation country:
Germany