Using accurate mass electrospray ionization-time-of-flight mass spectrometry with in-source collision-induced dissociation to sequence peptide mixtures.
J Chromatogr A
; 1020(1): 11-26, 2003 Dec 05.
Article
in En
| MEDLINE
| ID: mdl-14661753
Although data-dependent LC-MS-MS with database searching has become au courant for identifying proteins, the technique is constrained by duty-cycle inefficiency and the inability of most tandem mass analyzers to accurately measure peptide product ion masses. In this work, a novel approach is presented for simultaneous peptide fragmentation and accurate mass measurement using in-source collision-induced dissociation (CID) on electrospray ionization (ESI)-time-of-flight (TOF) MS. By employing internal mass reference compounds, mass measurement accuracy within +/-5 ppm for tryptic peptide precursors and +/-10 ppm for most sequence-specific product ions was consistently achieved. Analysis of a complex solution containing several digested protein standards did not adversely affect instrument performance.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Spectrometry, Mass, Electrospray Ionization
Language:
En
Journal:
J Chromatogr A
Year:
2003
Document type:
Article
Affiliation country:
United States
Country of publication:
Netherlands