A 21-amino acid peptide from the cysteine cluster II of the family D DNA polymerase from Pyrococcus horikoshii stimulates its nuclease activity which is Mre11-like and prefers manganese ion as the cofactor.

Shen, Yulong; Tang, Xiao-Feng; Yokoyama, Hideshi; Matsui, Eriko; Matsui, Ikuo

Shen, Yulong; Tang, Xiao-Feng; Yokoyama, Hideshi; Matsui, Eriko; Matsui, Ikuo.

Affiliation

Shen Y; Biological Information Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305, Japan.

Nucleic Acids Res ; 32(1): 158-68, 2004.

Article in En | MEDLINE | ID: mdl-14704353

Subject(s)

Archaeal Proteins/chemistry; Coenzymes/metabolism; DNA-Directed DNA Polymerase/chemistry; DNA-Directed DNA Polymerase/metabolism; Endodeoxyribonucleases/chemistry; Exodeoxyribonucleases/chemistry; Manganese/metabolism; Peptide Fragments/pharmacology; Pyrococcus horikoshii/enzymology; Amino Acid Sequence; Archaeal Proteins/metabolism; Binding Sites; Catalysis/drug effects; Catalytic Domain; Coenzymes/pharmacology; Cysteine/analysis; DNA, Single-Stranded/genetics; DNA, Single-Stranded/metabolism; DNA-Directed DNA Polymerase/genetics; Endodeoxyribonucleases/metabolism; Exodeoxyribonucleases/metabolism; Manganese/pharmacology; Models, Molecular; Molecular Sequence Data; Peptide Fragments/chemistry; Peptide Fragments/genetics; Peptide Fragments/metabolism; Protein Subunits/chemistry; Protein Subunits/metabolism; Pyrococcus horikoshii/genetics; Sequence Deletion/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Fragments / Coenzymes / Archaeal Proteins / Pyrococcus horikoshii / DNA-Directed DNA Polymerase / Endodeoxyribonucleases / Exodeoxyribonucleases / Manganese Type of study: Prognostic_studies Language: En Journal: Nucleic Acids Res Year: 2004 Document type: Article Affiliation country: Japan Country of publication: United kingdom

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