A fluorescence-based high-throughput screening assay for inhibitors of human immunodeficiency virus-1 reverse transcriptase-associated ribonuclease H activity.

Parniak, Michael A; Min, Kyung-Lyum; Budihas, Scott R; Le Grice, Stuart F J; Beutler, John A

Parniak, Michael A; Min, Kyung-Lyum; Budihas, Scott R; Le Grice, Stuart F J; Beutler, John A.

Affiliation

Parniak MA; Department of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. parniakm@msx.dept-med.pitt.edu

Anal Biochem ; 322(1): 33-9, 2003 Nov 01.

Article in En | MEDLINE | ID: mdl-14705777

Subject(s)

HIV Reverse Transcriptase/antagonists & inhibitors; HIV-1/enzymology; Ribonuclease H/metabolism; Base Sequence; DNA Probes/metabolism; Drug Evaluation, Preclinical; Enzyme Inhibitors/pharmacology; Fluorescein; Fluorescence Resonance Energy Transfer; HIV Reverse Transcriptase/metabolism; HIV-1/drug effects; Humans; Molecular Sequence Data; RNA Probes/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: HIV-1 / Ribonuclease H / HIV Reverse Transcriptase Type of study: Diagnostic_studies / Risk_factors_studies / Screening_studies Limits: Humans Language: En Journal: Anal Biochem Year: 2003 Document type: Article Affiliation country: United States Country of publication: United States

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