In vivo fluorescence microscopy of neuronal activity in three dimensions by use of voltage-sensitive dyes.
Opt Lett
; 29(1): 71-3, 2004 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-14719664
ABSTRACT
We report in vivo imaging of neuronal electrical activity from superficial layers of the mouse barrel cortex. The measurements have approximately 16-microm spatial and 3-ms temporal resolution and reach depths of 150 microm below the cortical surface. The depth-dependent differential-fluorescence optical sections of activity are consistent with known cortical architecture and represent an important step toward in vivo measurement of functioning complex neural networks. Our observations employ a custom gradient-index lens probe and voltage-sensitive dye fluorescence; the use of epi-illumination rather than dark-field illumination provides the dramatic signal-to-noise improvement necessary for fast three-dimensional imaging.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Somatosensory Cortex
/
Styrenes
/
Imaging, Three-Dimensional
/
Coloring Agents
/
Neurons
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Opt Lett
Year:
2004
Document type:
Article
Affiliation country:
United States